Molecular Cloning of the Vitellogenin Gene and the Effects of Vitellogenin Protein Expression on the Physiology of Harmonia axyridis (Coleoptera: Coccinellidae).

ABSTRACT: Vitellogenin (Vg), the main egg storage protein precursor, plays an integral role in many oviparous animals, including Harmonia axyridis, an important agent for the biological control of many insect pests. In this study, the full-length Vg gene of was cloned. The open reading frame (ORF) of H. axyridis Vg cDNA is 5,403 bp in length and encodes 1,800 amino acids, with a predicted molecular mass of 211.88 KDa (accession number in NCBI: KX442718). Recombinant protein (18 kDa) expressed by the cloned Vg gene was characterized, and the effects of the expression of this protein on the physiology of H. axyridis were investigated. We found that Vg fragment significantly increased the egg production of H. axyridis. Furthermore, we also found that the activities of trypsin and lipase in H. axyridis

SUBMITTER: Zhang T 

PROVIDER: S-EPMC5654974 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature