Comparative proteomic analysis of virulent and avirulent strains of Toxoplasma gondii reveals strain-specific patterns.

ABSTRACT: Research exploring the proteome of Toxoplasma gondii oocysts has gained momentum over the past few years. However, little is known about the oocyst's protein repertoires that contribute to differential virulence among T. gondii strains. Here, we used isobaric tag for relative and absolute quantitation-based proteomic analysis of oocysts of two T. gondii strains exhibiting the virulent PYS (ToxoDB#9) phenotype versus the less virulent PRU (Type II, ToxoDB#1) phenotype. Our aim was to determine protein expression patterns that contribute to the virulence of a particular phenotype. A total of 2,551 proteins were identified, of which 374 were differentially expressed proteins (DEPs) (|log₂ fold change| ≥ 0.58 and P < 0.05). DEPs included 192 increased and

SUBMITTER: Zhou DH 

PROVIDER: S-EPMC5655214 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature