Knockout of toll-like receptor impairs nerve regeneration after a crush injury.

ABSTRACT:

Background

Toll-like receptors (TLRs) are involved in the initiation of Schwann cell activation and subsequent recruitment of macrophages for clearance of degenerated myelin and neuronal debris after nerve injury. The present study was designed to investigate the regenerative outcome and expression of myelination-related factors in Tlr-knockout mice following a sciatic nerve crush injury.

Materials and methods

A standard sciatic nerve crush injury, induced by applying constant pressure to the nerve with a No. 5 jeweler's forceps for 30 s, was performed in C57BL/6, Tlr2^-/- , Tlr3^-/- , Tlr4^-/- , Tlr5^-/- , and Tlr7^-/- mice. Quantitative histomorphometric analysis of toluidine blue-s

SUBMITTER: Hsieh CH 

PROVIDER: S-EPMC5655236 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature