SG2NA is a regulator of endoplasmic reticulum (ER) homeostasis as its depletion leads to ER stress.

ABSTRACT: SG2NA belongs to a three-member striatin subfamily of WD40 repeat superfamily of proteins. It has multiple protein-protein interaction domains involved in assembling supramolecular signaling complexes. Earlier, we had demonstrated that there are at least five variants of SG2NA generated by alternative splicing, intron retention, and RNA editing. Such versatile and dynamic mode of regulation implicates it in tissue development. In order to shed light on its role in cell physiology, total proteome analysis was performed in NIH3T3 cells depleted of 78 kDa SG2NA, the only isoform expressing therein. A number of ER stress markers were among those modulated after knockdown of SG2NA. In cells treated with the ER stressors thapsigargin and tunicamycin, expression of SG2NA was increased at both mRN

SUBMITTER: Jain BP 

PROVIDER: S-EPMC5655373 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature