Kinetic characterization of Vibrio cholerae ApbE: Substrate specificity and regulatory mechanisms.

ABSTRACT: ApbE is a member of a novel family of flavin transferases that incorporates flavin mononucleotide (FMN) to subunits of diverse respiratory complexes, which fulfill important homeostatic functions. In this work a detailed characterization of Vibrio cholerae ApbE physiologic activity, substrate specificity and pH dependency was carried out. The data obtained show novel characteristics of the regulation and function of this family. For instance, our experiments indicate that divalent cations are essential for ApbE function, and that the selectivity depends largely on size and the coordination sphere of the cation. Our data also show that ApbE regulation by pH, ADP and potassium is an important mechanism that enhances the adaptation, survival and colonization of V. cholerae in the small intest

SUBMITTER: Fang X 

PROVIDER: S-EPMC5655446 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature