Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution.

ABSTRACT: Secreted mixtures of Hypocrea jecorina cellulases are able to efficiently degrade cellulosic biomass to fermentable sugars at large, commercially relevant scales. H. jecorina Cel7A, cellobiohydrolase I, from glycoside hydrolase family 7, is the workhorse enzyme of the process. However, the thermal stability of Cel7A limits its use to processes where temperatures are no higher than 50 °C. Enhanced thermal stability is desirable to enable the use of higher processing temperatures and to improve the economic feasibility of industrial biomass conversion. Here, we enhanced the thermal stability of Cel7A through directed evolution. Sites with increased thermal stability properties were combined, and a Cel7A variant (FCA398) was obtained, which exhibited a 10.4 °C increase in T

SUBMITTER: Goedegebuur F 

PROVIDER: S-EPMC5655518 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature