Processive searching ability varies among members of the gap-filling DNA polymerase X family.

ABSTRACT: DNA repair proteins must locate rare damaged sites within the genome. DNA polymerase β (Pol β), a member of the DNA polymerase X family that is involved in base excision repair, uses a processive hopping search mechanism to locate substrates. This effectively enhances its search footprint on DNA, increasing the probability of locating damaged sites. Processive searching has been reported or proposed for many DNA-binding proteins, raising the question of how widespread or specific to certain enzymes the ability to perform this function is. To provide insight into this question, we compared the ability of three homologous DNA Pol X family members to perform a processive search for 1-nucleotide gaps in DNA using a previously developed biochemical assay. We found that at near-predicted physiol

SUBMITTER: Howard MJ 

PROVIDER: S-EPMC5655522 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature