Chromatin status and transcription factor binding to gonadotropin promoters in gonadotrope cell lines.

ABSTRACT:

Background

Proper expression of key reproductive hormones from gonadotrope cells of the pituitary is required for pubertal onset and reproduction. To further our understanding of the molecular events taking place during embryonic development, leading to expression of the glycoproteins luteinizing hormone (LH) and follicle-stimulating hormone (FSH), we characterized chromatin structure changes, imparted mainly by histone modifications, in model gonadotrope cell lines.

Methods

We evaluated chromatin status and gene expression profiles by chromatin immunoprecipitation assays, DNase sensitivity assay, and RNA sequencing in three developmentally staged gonadotrope cell lines, αT1-1 (progenitor, expressing Cga), αT3-1 (immature, expressing Cga and Gnrhr), and LβT2 (mature, express

SUBMITTER: Xie H 

PROVIDER: S-EPMC5655979 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature