Binding patterns of homo-peptides on bare magnetic nanoparticles: insights into environmental dependence.

ABSTRACT: Magnetic nanoparticles (MNP) are intensively investigated for applications in nanomedicine, catalysis and biotechnology, where their interaction with peptides and proteins plays an important role. However, the characterisation of the interaction of individual amino acids with MNP remains challenging. Here, we classify the affinity of 20 amino acid homo-hexamers to unmodified iron oxide nanoparticles using peptide arrays in a variety of conditions as a basis to identify and rationally design selectively binding peptides. The choice of buffer system is shown to strongly influence the availability of peptide binding sites on the MNP surface. We find that under certain buffer conditions peptides of different charges can bind the MNP and that the relative strength of the interactions can be mod

SUBMITTER: Blank-Shim SA 

PROVIDER: S-EPMC5656586 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature