A simple surface plasmon resonance biosensor for detection of PML/RARα based on heterogeneous fusion gene-triggered nonlinear hybridization chain reaction.

ABSTRACT: In this work, a simple and enzyme-free surface plasmon resonance (SPR) biosensing strategy has been developed for highly sensitive detection of two major PML/RARα (promyelocytic leukemia, retinoic acid receptor alpha) subtypes based on the heterogeneous fusion gene-triggered nonlinear hybridization chain reaction (HCR). On the gold chip surface, the cascade self-assembly process is triggered after the introduction of PML/RARα. The different fragments of PML/RARα can specifically hybridize with capture probes (Cp) immobilized on the chip and the hybridization DNA₁ (H₁). Then, the nonlinear HCR is initiated by the complex of Cp-PML/RARα-H₁ with the introduction of two hybridization DNA chains (H₂ and H₃). As a result, a dendritic nanostr

SUBMITTER: Guo B 

PROVIDER: S-EPMC5656617 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature