Characterization of histone acylations links chromatin modifications with metabolism.

ABSTRACT: Over the last decade, numerous histone acyl post-translational modifications (acyl-PTMs) have been discovered, of which the functional significance is still under intense study. Here, we use high-resolution mass spectrometry to accurately quantify eight acyl-PTMs in vivo and after in vitro enzymatic assays. We assess the ability of seven histone acetyltransferases (HATs) to catalyze acylations on histones in vitro using short-chain acyl-CoA donors, proving that they are less efficient towards larger acyl-CoAs. We also observe that acyl-CoAs can acylate histones through non-enzymatic mechanisms. Using integrated metabolomic and proteomic approaches, we achieve high correlation (R ² > 0.99) between the abundance of acyl-CoAs and their corresponding acyl-PTMs. Moreover, we observe

SUBMITTER: Simithy J 

PROVIDER: S-EPMC5656686 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature