Netrin-4 Promotes Differentiation and Migration of Osteoblasts.

ABSTRACT:

Background/aim

While netrin-4 plays a vital role in the vascular system, the role of netrin-1 in osteoblast differentiation is not well understood. In this study we explored whether netrin-4 has functional roles in osteoblasts.

Materials and methods

Quantitative reverse-transcriptase polymerase chain reaction (PCR), RNA interference, the generation of plasmids, transfections, measurement of alkaline phosphatase activity, a mineralization assay, a migration assay and a cell proliferation assay were performed.

Results

Netrin-4 expression was up-regulated during osteoblast differentiation and an RNA interference experiment showed that small interfering RNA used to silence netrin-4 inhibited osteoblast differentiation. Recombinant mouse netrin-4 promoted alkaline phosphat

SUBMITTER: Enoki Y 

PROVIDER: S-EPMC5656851 | biostudies-literature | 2017 Sep-Oct

REPOSITORIES: biostudies-literature