Marburg virus-like particles by co-expression of glycoprotein and matrix protein in insect cells induces immune responses in mice.

ABSTRACT:

Background

Marburg virus (MARV) causes severe haemorrhagic fever in humans and nonhuman primates and has a high mortality rate. However, effective drugs or licensed vaccines are not currently available to control the outbreak and spread of this disease.

Methods

In this study, we generated MARV virus-like particles (VLPs) by co-expressing the glycoprotein (GP) and matrix protein (VP40) using the baculovirus expression system. MARV VLPs and three adjuvants, Poria cocos polysaccharide (PCP-II), poly(I:C) and aluminium hydroxide, were evaluated after intramuscular vaccination in mice.

Results

Murine studies demonstrated that vaccination with the MARV VLPs induce neutralizing antibodies and cellar immune responses. MARV VLPs and the PCP-II adjuvant group resulted in high t

SUBMITTER: Gai W 

PROVIDER: S-EPMC5657058 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature