Development of a total colectomy and ileorectal anastomosis rat model to evaluate colonic metaplasia.

ABSTRACT:

Background

Ulcerative colitis is an idiopathic inflammatory condition of the colon that may require surgical intervention including proctocolectomy and either ileal pouch-anal anastomosis or in the pediatric population, low ileorectal anastomosis (IRA). Often, subsequent physiologic alteration (or colonic metaplasia) occurs in the anastomosed small bowel that includes changes in mucin content, villous blunting, and increased expression of WNT5A, a marker of colonic crypt regeneration. We developed a rat low IRA model to assess and study the development of colonic metaplasia.

Materials and methods

We subjected male Sprague-Dawley rats (n = 17) to total colectomy and low IRA surgery and evaluated healing periodically by endoscopic evaluation. The ileum upstream of the anastomo

SUBMITTER: Schieffer KM 

PROVIDER: S-EPMC5657454 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature