RNA-seq transcriptome analysis of formalin fixed, paraffin-embedded canine meningioma.

ABSTRACT: Meningiomas are the most commonly reported primary intracranial tumor in dogs and humans and between the two species there are similarities in histology and biologic behavior. Due to these similarities, dogs have been proposed as models for meningioma pathobiology. However, little is known about specific pathways and individual genes that are involved in the development and progression of canine meningioma. In addition, studies are lacking that utilize RNAseq to characterize gene expression in clinical cases of canine meningioma. The primary objective of this study was to develop a technique for which high quality RNA can be extracted from formalin-fixed, paraffin embedded tissue and then used for transcriptome analysis to determine patterns of gene expression. RNA was extracted from thirt

SUBMITTER: Grenier JK 

PROVIDER: S-EPMC5658167 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature