Organelle membrane derived patches: reshaping classical methods for new targets.

ABSTRACT: Intracellular ion channels are involved in multiple signaling processes, including such crucial ones as regulation of cellular motility and fate. With 95% of the cellular membrane belonging to intracellular organelles, it is hard to overestimate the importance of intracellular ion channels. Multiple studies have been performed on these channels over the years, however, a unified approach allowing not only to characterize their activity but also to study their regulation by partner proteins, analogous to the patch clamp "golden standard", is lacking. Here, we present a universal approach that combines the extraction of intracellular membrane fractions with the preparation of patchable substrates that allows to characterize these channels in endogenous protein environment and to study their

SUBMITTER: Shapovalov G 

PROVIDER: S-EPMC5658434 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature