An ArsR/SmtB family member regulates arsenic resistance genes unusually arranged in Thermus thermophilus HB27.

ABSTRACT: Arsenic resistance is commonly clustered in ars operons in bacteria; main ars operon components encode an arsenate reductase, a membrane extrusion protein, and an As-sensitive transcription factor. In the As-resistant thermophile Thermus thermophilus HB27, genes encoding homologues of these proteins are interspersed in the chromosome. In this article, we show that two adjacent genes, TtsmtB, encoding an ArsR/SmtB transcriptional repressor and, TTC0354, encoding a Zn²⁺ /Cd²⁺ -dependent membrane ATPase are involved in As resistance; differently from characterized ars operons, the two genes are transcribed from dedicated promoters upstream of their respective genes, whose expression is differentially regulated at transcriptional level. Mutants defective in TtsmtB or TTC0

SUBMITTER: Antonucci I 

PROVIDER: S-EPMC5658604 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature