Metabolic and stress responses of Acinetobacter oleivorans DR1 during long-chain alkane degradation.

ABSTRACT: Acinetobacter oleivorans DR1 can utilize C₁₂ -C₃₀ alkanes as a sole carbon source but not short-chain alkanes (C₆ , C₁₀ ). Two copies of each alkB-, almA- and ladA-type alkane hydroxylase (AH) are present in the genome of DR1 cells. Expression and mutational analyses of AHs showed that alkB1 and alkB2 are the major AH-encoding genes under C₁₂ -C₃₀ , and the roles of other almA- and ladA genes are negligible. Our data suggested that AlkB1 is responsible for long-chain alkane utilization (C₂₄ -C₂₆ ), and AlkB2 is important for medium-chain alkane (C₁₂ -C₁₆ ) metabolism. Phylogenetic analyses revealed large incongruities between phylogenies of 16S rRNA and each AH gene, which implies

SUBMITTER: Park C 

PROVIDER: S-EPMC5658608 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature