Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation.

ABSTRACT:

Background

The pollen tube (PT) serves as a model system for investigating plant cell growth and morphogenesis. Ultrastructural studies are indispensable to complement data from physiological and genetic analyses, yet an effective method is lacking for PTs of the model plant Arabidopsis thaliana.

Methods

Here, we present reliable approaches for ultrastructural studies of Arabidopsis PTs, as well as an efficient technique for immunogold detection of cell wall epitopes. Using different fixation and embedding strategies, we show the amount of PT ultrastructural details that can be obtained by the different methods.

Results

Dozens of cross-sections can be obtained simultaneously by the approach, which facilitates and shortens the time for evaluation. In addition to in vit

SUBMITTER: Ndinyanka Fabrice T 

PROVIDER: S-EPMC5658917 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature