Restoring the quantity and quality of elderly human mesenchymal stem cells for autologous cell-based therapies.

ABSTRACT:

Background

Degenerative diseases are a major public health concern for the aging population and mesenchymal stem cells (MSCs) have great potential for treating many of these diseases. However, the quantity and quality of MSCs declines with aging, limiting the potential efficacy of autologous MSCs for treating the elderly population.

Methods

Human bone marrow (BM)-derived MSCs from young and elderly donors were obtained and characterized using standard cell surface marker criteria (CD73, CD90, CD105) as recommended by the International Society for Cellular Therapy (ISCT). The elderly MSC population was isolated into four subpopulations based on size and stage-specific embryonic antigen-4 (SSEA-4) expression using fluorescence-activated cell sorting (FACS), and subpopulations

SUBMITTER: Block TJ 

PROVIDER: S-EPMC5658952 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature