Establishment and characterization of a novel murine model of pancreatic cancer cachexia.

ABSTRACT:

Background

Cachexia is a complex metabolic and behavioural syndrome lacking effective therapies. Pancreatic ductal adenocarcinoma (PDAC) is one of the most important conditions associated with cachexia, with >80% of PDAC patients suffering from the condition. To establish the cardinal features of a murine model of PDAC-associated cachexia, we characterized the effects of implanting a pancreatic tumour cell line from a syngeneic C57BL/6 KRAS^G12D P53^R172H Pdx-Cre^+/+ (KPC) mouse.

Methods

Male and female C57BL/6 mice were inoculated subcutaneously, intraperitoneally, or orthotopically with KPC tumour cells. We performed rigorous phenotypic, metabolic, and behavioural analysis of animals over the course of tumour development.

Results

All ro

SUBMITTER: Michaelis KA 

PROVIDER: S-EPMC5659050 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature