Metabolic modeling to identify engineering targets for Komagataella phaffii: The effect of biomass composition on gene target identification.

ABSTRACT: Genome-scale metabolic models are valuable tools for the design of novel strains of industrial microorganisms, such as Komagataella phaffii (syn. Pichia pastoris). However, as is the case for many industrial microbes, there is no executable metabolic model for K. phaffiii that confirms to current standards by providing the metabolite and reactions IDs, to facilitate model extension and reuse, and gene-reaction associations to enable identification of targets for genetic manipulation. In order to remedy this deficiency, we decided to reconstruct the genome-scale metabolic model of K. phaffii by reconciling the extant models and performing extensive manual curation in order to construct an executable model (Kp.1.0) that conforms to current standards. We then used this model to study the effe

SUBMITTER: Cankorur-Cetinkaya A 

PROVIDER: S-EPMC5659126 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature