Time-lapsed, large-volume, high-resolution intravital imaging for tissue-wide analysis of single cell dynamics.

ABSTRACT: Pathologists rely on microscopy to diagnose disease states in tissues and organs. They utilize both high-resolution, high-magnification images to interpret the staining and morphology of individual cells, as well as low-magnification overviews to give context and location to these cells. Intravital imaging is a powerful technique for studying cells and tissues in their native, live environment and can yield sub-cellular resolution images similar to those used by pathologists. However, technical limitations prevent the straightforward acquisition of low-magnification images during intravital imaging, and they are hence not typically captured. The serial acquisition, mosaicking, and stitching together of many high-resolution, high-magnification fields of view is a technique that overcomes th

SUBMITTER: Entenberg D 

PROVIDER: S-EPMC5659295 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature