A practical approach to pancreatic cancer immunotherapy using resected tumor lysate vaccines processed to express α-gal epitopes.

ABSTRACT:

Objectives

Single-agent immunotherapy is ineffective against poorly immunogenic cancers, including pancreatic ductal adenocarcinoma (PDAC). The aims of this study were to demonstrate the feasibility of production of novel autologous tumor lysate vaccines from resected PDAC tumors, and verify vaccine safety and efficacy.

Methods

Fresh surgically resected tumors obtained from human patients were processed to enzymatically synthesize α-gal epitopes on the carbohydrate chains of membrane glycoproteins. Processed membranes were analyzed for the expression of α-gal epitopes and the binding of anti-Gal, and vaccine efficacy was assessed in vitro and in vivo.

Results

Effective synthesis of α-gal epitopes was demonstrated after processing of PDAC tumor lysates from 10 differen

SUBMITTER: Furukawa K 

PROVIDER: S-EPMC5659602 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature