Cardioprotective Effects of HSP72 Administration on Ischemia-Reperfusion Injury.

ABSTRACT:

Background

Although early reperfusion is the most desirable intervention after ischemic myocardial insult, it may add to damage through oxidative stress.

Objectives

This study investigated the cardioprotective effects of a single intravenous dose of heat shock protein-72 (HSP72) coupled to a single-chain variable fragment (Fv) of monoclonal antibody 3E10 (3E10Fv) in a rabbit ischemia-reperfusion model. The Fv facilitates rapid transport of HSP72 into cells, even with intact membranes.

Methods

A left coronary artery occlusion (40 min) reperfusion (3 h) model was used in 31 rabbits. Of these, 12 rabbits received the fusion protein (Fv-HSP72) intravenously. The remaining 19 control rabbits received a molar equivalent of 3E10Fv alone (n = 6), HSP72 alone (n = 6), or phosp

SUBMITTER: Tanimoto T 

PROVIDER: S-EPMC5659834 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature