Fast, ultra-trace detection of juvenile hormone III from mosquitoes using mass spectrometry.

ABSTRACT: In the present work, a new protocol for fast separation and quantification of JH III from biological samples using liquid chromatography coupled to electrospray tandem mass spectrometry is described. In particular, the proposed protocol improves existing methodologies by combining a limited number of sample preparation steps with fast LC-MS/MS detection, providing lower limits of detection and demonstrated matrix effect control, together with high inter and intraday reproducibility. A limit of detection of 8pg/mL (0.32pg on column) was achieved, representing a 15-fold gain in sensitivity with respect to previous LC-MS based protocols. The performance of the LC-MS/MS protocol is comparable to previously described JH III quantitation protocol based on fluorescence detection, with the added a

SUBMITTER: Ramirez CE 

PROVIDER: S-EPMC5659872 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature