Mechanisms underlying induction of allergic sensitization by Pru p 3.

ABSTRACT:

Background

Recently, the nature of the lipid-ligand of Pru p 3, one of the most common plant food allergens in southern Europe, has been identified as a derivative of the alkaloid camptothecin bound to phytosphingosine. However, the origin of its immunological activity is still unknown.

Objective

We sought to evaluate the role of the Pru p 3 lipid-ligand in the immunogenic activity of Pru p 3.

Methods

In vitro cultures of different cell types (monocyte-derived dendritic cells [moDCs], PBMCs [peripheral blood mononuclear cells] and epithelial and iNKT-hybridoma cell lines) have been used to determine the immunological capacity of the ligand, by measuring cell proliferation, maturation markers and cytokine production. To study the capacity of the lipid-ligand to promote

SUBMITTER: Tordesillas L 

PROVIDER: S-EPMC5659917 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature