Functionalization of microparticles with mineral coatings enhances non-viral transfection of primary human cells.

ABSTRACT: Gene delivery to primary human cells is a technology of critical interest to both life science research and therapeutic applications. However, poor efficiencies in gene transfer and undesirable safety profiles remain key limitations in advancing this technology. Here, we describe a materials-based approach whereby application of a bioresorbable mineral coating improves microparticle-based transfection of plasmid DNA lipoplexes in several primary human cell types. In the presence of these mineral-coated microparticles (MCMs), we observed up to 4-fold increases in transfection efficiency with simultaneous reductions in cytotoxicity. We identified mechanisms by which MCMs improve transfection, as well as coating compositions that improve transfection in three-dimensional cell constructs. The

SUBMITTER: Khalil AS 

PROVIDER: S-EPMC5660152 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature