Fluorescence-based cell-specific detection for laser-capture microdissection in human brain.

ABSTRACT: Cell-specific molecular investigations of the human brain are essential for understanding the neurobiology of diseases, but are hindered by postmortem conditions and technical challenges. To address these issues we developed a multi-label fluorescence in situ hybridization protocol and a novel optical filter device to identify cell types and control for tissue autofluorescence. We show that these methods can be used with laser-capture microdissection for human brain tissue cell-specific molecular analysis.

SUBMITTER: Rocco BR 

PROVIDER: S-EPMC5660154 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature