Glycolipid-peptide conjugate vaccines enhance CD8⁺ T cell responses against human viral proteins.

ABSTRACT: An important goal of vaccination against viruses and virus-driven cancers is to elicit cytotoxic CD8⁺ T cells specific for virus-derived peptides. CD8⁺ T cell responses can be enhanced by engaging help from natural killer T (NKT) cells. We have produced synthetic vaccines that induce strong peptide-specific CD8⁺ T cell responses in vivo by incorporating an NKT cell-activating glycolipid. Here we examine the effect of a glycolipid-peptide conjugate vaccine incorporating an NKT cell-activating glycolipid linked to an MHC class I-restricted peptide from a viral antigen in human peripheral blood mononuclear cells. The vaccine induces CD1d-dependent activation of human NKT cells following enzymatic cleavage, activates human dendritic cells in an NKT-cell depende

SUBMITTER: Speir M 

PROVIDER: S-EPMC5660197 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature