Dual Biomembrane Force Probe enables single-cell mechanical analysis of signal crosstalk between multiple molecular species.

ABSTRACT: Conventional approaches for studying receptor-mediated cell signaling, such as the western blot and flow cytometry, are limited in three aspects: 1) The perturbing preparation procedures often alter the molecules from their native state on the cell; 2) Long processing time before the final readout makes it difficult to capture transient signaling events (<1 min); 3) The experimental environments are force-free, therefore unable to visualize mechanical signals in real time. In contrast to these methods in biochemistry and cell biology that are usually population-averaged and non-real-time, here we introduce a novel single-cell based nanotool termed dual biomembrane force probe (dBFP). The dBFP provides precise controls and quantitative readouts in both mechanical and chemical terms, which i

SUBMITTER: Ju L 

PROVIDER: S-EPMC5660210 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature