Single-virion sequencing of lamivudine-treated HBV populations reveal population evolution dynamics and demographic history.

ABSTRACT:

Background

Viral populations are complex, dynamic, and fast evolving. The evolution of groups of closely related viruses in a competitive environment is termed quasispecies. To fully understand the role that quasispecies play in viral evolution, characterizing the trajectories of viral genotypes in an evolving population is the key. In particular, long-range haplotype information for thousands of individual viruses is critical; yet generating this information is non-trivial. Popular deep sequencing methods generate relatively short reads that do not preserve linkage information, while third generation sequencing methods have higher error rates that make detection of low frequency mutations a bioinformatics challenge. Here we applied BAsE-Seq, an Illumina-based single-virion sequenc

SUBMITTER: Zhu YO 

PROVIDER: S-EPMC5660452 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature