Dynamic changes in eIF4F-mRNA interactions revealed by global analyses of environmental stress responses.

ABSTRACT:

Background

Translation factors eIF4E and eIF4G form eIF4F, which interacts with the messenger RNA (mRNA) 5' cap to promote ribosome recruitment and translation initiation. Variations in the association of eIF4F with individual mRNAs likely contribute to differences in translation initiation frequencies between mRNAs. As translation initiation is globally reprogrammed by environmental stresses, we were interested in determining whether eIF4F interactions with individual mRNAs are reprogrammed and how this may contribute to global environmental stress responses.

Results

Using a tagged-factor protein capture and RNA-sequencing (RNA-seq) approach, we have assessed how mRNA associations with eIF4E, eIF4G1 and eIF4G2 change globally in response to three defined stresses that each

SUBMITTER: Costello JL 

PROVIDER: S-EPMC5660459 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature