Isotopically nonstationary ¹³C flux analysis of cyanobacterial isobutyraldehyde production.

ABSTRACT: We applied isotopically nonstationary ¹³C metabolic flux analysis (INST-MFA) to compare the pathway fluxes of wild-type (WT) Synechococcus elongatus PCC 7942 to an engineered strain (SA590) that produces isobutyraldehyde (IBA). The flux maps revealed a potential bottleneck at the pyruvate kinase (PK) reaction step that was associated with diversion of flux into a three-step PK bypass pathway involving the enzymes PEP carboxylase (PEPC), malate dehydrogenase (MDH), and malic enzyme (ME). Overexpression of pk in SA590 led to a significant improvement in IBA specific productivity. Single-gene overexpression of the three enzymes in the proposed PK bypass pathway also led to improvements in IBA production, although to a lesser extent than pk overexpression. Combinatorial overexpressi

SUBMITTER: Jazmin LJ 

PROVIDER: S-EPMC5660605 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature