Photoreceptor cells produce inflammatory products that contribute to retinal vascular permeability in a mouse model of diabetes.

ABSTRACT:

Aims/hypothesis

Recent studies suggest that photoreceptor cells produce mediators or products that contribute to retinal capillary damage in diabetes. The purpose of this study was to determine if photoreceptor cells release soluble factors that contribute to retinal vascular permeability in diabetes.

Methods

To assess retinal vascular leakage, a streptozotocin-induced mouse model of diabetes, with hyperglycaemia for 8 months, and age-matched control mice, were injected with FITC-BSA. Fluorescence microscopy was used to detect leakage of FITC-BSA from the retinal vasculature into the neural retina. Ex vivo and in vitro experiments were performed to determine if photoreceptor cells released products that directly increased retinal endothelial cell permeability or cell death.

SUBMITTER: Tonade D 

PROVIDER: S-EPMC5660634 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature