Optimized production and isolation of antibacterial agent from marine Aspergillus flavipes against Vibrio harveyi.

ABSTRACT: Statistical methodologies, including Plackett-Burman design and Box-Behnken design, were employed to optimize the fermentation conditions for the production of active substances against aquatic pathogen Vibrio harveyi by marine-derived Aspergillus flavipes strain HN4-13. The optimal crucial fermentation values for maximum production of active substances against V. harveyi were obtained as follows: X₁ (peptone) = 0.3%, X₂ (KCl) = 0.25%, and X₃ (inoculum size) = 4.5%. The predicted diameter of inhibitory zone against V. harveyi was 23.39 mm, and the practical value reached 23.71 ± 0.98 mm with a 62.3% increase. Bioassay-guided fractionation resulted in the acquisition of two compounds whose struct

SUBMITTER: Guo L 

PROVIDER: S-EPMC5661027 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature