Excess cholesterol inhibits glucose-stimulated fusion pore dynamics in insulin exocytosis.

ABSTRACT: Type 2 diabetes is caused by defects in both insulin sensitivity and insulin secretion. Glucose triggers insulin secretion by causing exocytosis of insulin granules from pancreatic β-cells. High circulating cholesterol levels and a diminished capacity of serum to remove cholesterol from β-cells are observed in diabetic individuals. Both of these effects can lead to cholesterol accumulation in β-cells and contribute to β-cell dysfunction. However, the molecular mechanisms by which cholesterol accumulation impairs β-cell function remain largely unknown. Here, we used total internal reflection fluorescence microscopy to address, at the single-granule level, the role of cholesterol in regulating fusion pore dynamics during insulin exocytosis. We focused particularly on the effects of cholester

SUBMITTER: Xu Y 

PROVIDER: S-EPMC5661106 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature