Targeting YAP in malignant pleural mesothelioma.

ABSTRACT: Malignant mesothelioma is an aggressive cancer that is resistant to current therapy. The poor prognosis of mesothelioma has been associated with elevated Yes-associated protein (YAP) activity. In this study, we evaluated the effect of targeting YAP in mesothelioma. First, we comprehensively studied YAP activity in five mesothelioma cell lines (211H, H2052, H290, MS-1 and H2452) and one normal mesothelial cell line (LP9). We found decreased phospho-YAP to YAP protein ratio and consistently increased GTIIC reporter activity in 211H, H2052 and H290 compared to LP9. The same three cell lines (IC₅₀ s < 1 μM) were more sensitive than LP9 (IC₅₀ = 3.5 μM) to the YAP/TEAD inhibitor verteporfin. We also found that verteporfin significantly reduced YAP protein level, mRNA levels

SUBMITTER: Zhang WQ 

PROVIDER: S-EPMC5661117 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature