The EF-1α promoter maintains high-level transgene expression from episomal vectors in transfected CHO-K1 cells.

ABSTRACT: In our previous study, we demonstrated that episomal vectors based on the characteristic sequence of matrix attachment regions (MARs) and containing the cytomegalovirus (CMV) promoter allow transgenes to be maintained episomally in Chinese hamster ovary (CHO) cells. However, the transgene expression was unstable and the number of copies was low. In this study, we focused on enhancers, various promoters and promoter variants that could improve the transgene expression stability, expression magnitude (level) and the copy number of a MAR-based episomal vector in CHO-K1 cells. In comparison with the CMV promoter, the eukaryotic translation elongation factor 1 α (EF-1α, gene symbol EEF1A1) promoter increased the transfection efficiency, the transgene expression, the proportion of expression-pos

SUBMITTER: Wang X 

PROVIDER: S-EPMC5661254 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature