Lack of association between rrl and erm(41) mutations and clarithromycin resistance in Mycobacterium abscessus complex.

ABSTRACT:

Background

Mycobacterium abscessus complex (MABC) includes species with high resistance rates among mycobacterial pathogens. In fact, MABC infections may not respond to clarithromycin treatment, which has historically been very effective against MABC infection. Molecular markers have been proposed to detect both acquired (rrl polymorphisms) and inducible (erm(41) polymorphisms) clarithromycin resistance in MABC isolates.

Objectives

This study aimed to evaluate the susceptibility profile and molecular markers of clarithromycin resistance in MABC.

Methods

The clarithromycin susceptibility profile was determined by broth microdilution with reads on days 3, 5, 7 and 14. Mutations in the rrl and erm(41) genes were evaluated by polymerase chain reaction (PCR) using specific

SUBMITTER: Carneiro MDS 

PROVIDER: S-EPMC5661901 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature