Going deep into protein secondary structure with synchrotron radiation circular dichroism spectroscopy.

ABSTRACT: Circular dichroism (CD) spectroscopy is a fast, powerful, well-established, and widely used analytical technique in the biophysical and structural biology community to study protein secondary structure and to track changes in protein conformation in different environments. The use of the intense light of a synchrotron beam as the light source for collecting CD measurements has emerged as an enhanced method, known as synchrotron radiation circular dichroism (SRCD) spectroscopy, that has several advantages over the conventional CD method, including a significant spectral range extension for data collection, deeper access to the lower limit (cut-off) of conventional CD spectroscopy, an improved signal-to-noise ratio to increase accuracy in the measurements, and the possibility to collect meas

SUBMITTER: Kumagai PS 

PROVIDER: S-EPMC5662050 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature