Genetically defined elevated homocysteine levels do not result in widespread changes of DNA methylation in leukocytes.

ABSTRACT:

Background

DNA methylation is affected by the activities of the key enzymes and intermediate metabolites of the one-carbon pathway, one of which involves homocysteine. We investigated the effect of the well-known genetic variant associated with mildly elevated homocysteine: MTHFR 677C>T independently and in combination with other homocysteine-associated variants, on genome-wide leukocyte DNA-methylation.

Methods

Methylation levels were assessed using Illumina 450k arrays on 9,894 individuals of European ancestry from 12 cohort studies. Linear-mixed-models were used to study the association of additive MTHFR 677C>T and genetic-risk score (GRS) based on 18 homocysteine-associated SNPs, with genome-wide methylation.

Results

Meta-analysis revealed that the MTHFR 677C>T va

SUBMITTER: Mandaviya PR 

PROVIDER: S-EPMC5662081 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature