A novel multicolor flow-cytometry application for quantitative detection of receptors on vascular smooth muscle cells.

ABSTRACT: There is a need to develop new techniques for quantitative measurement of receptors expression on particular vasculature cells types. Here, we describe and demonstrate a novel method to measure quantitatively and simultaneously the expression of endothelin B receptor (ETB) on vascular smooth muscle cells (VSMC). We isolated cells from male rat tissues such as: brain pial, brain intraparenchymal and retina vessels. To analyze solid tissues, a single-cell suspension was prepared by a combined mechanic and enzymatic process. The cells were stained with Fixable Viability Dye, followed by fixation, permeabilization and antibodies staining. The expression of ETB receptors on VSMC was measured by flow-cytometry and visualized by fluorescence microscopy. We obtained a high percentage of viable cel

SUBMITTER: Radziwon-Balicka A 

PROVIDER: S-EPMC5662092 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature