Evaluation of an amide-based stationary phase for supercritical fluid chromatography.

ABSTRACT: A relatively new stationary phase containing a polar group embedded in a hydrophobic backbone (i.e., ACE®C18-amide) was evaluated for use in supercritical fluid chromatography. The amide-based column was compared with columns packed with bare silica, C18 silica, and a terminal-amide silica phase. The system was held at supercritical pressure and temperature with a mobile phase composition of CO2 and methanol as cosolvent. The linear solvation energy relationship model was used to evaluate the behavior of these stationary phases, relating the retention factor of selected probes to specific chromatographic interactions. A five-component test mixture, consisting of a group of drug-like molecules was separated isocratically. The results show that the C18 -amide stationary phase provided a comb

SUBMITTER: Borges-Munoz AC 

PROVIDER: S-EPMC5662104 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature