Endogenous miRNA in the green alga Chlamydomonas regulates gene expression through CDS-targeting.

ABSTRACT: MicroRNAs (miRNAs) are 21-24-nucleotide RNAs present in many eukaryotes that regulate gene expression as part of the RNA-induced silencing complex. The sequence identity of the miRNA provides the specificity to guide the silencing effector Argonaute (AGO) protein to target mRNAs via a base-pairing process ¹ . The AGO complex promotes translation repression and/or accelerated decay of this target mRNA ² . There is overwhelming evidence both in vivo and in vitro that translation repression plays a major role ^3-7 . However, there has been controversy about which of these three mechanisms is more significant in vivo, especially when effects of miRNA on endogenous genes cannot be faithfully represented by reporter systems in which, at least in metazoans, the obs

SUBMITTER: Chung BY 

PROVIDER: S-EPMC5662147 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature