Meiotic crossovers are associated with open chromatin and enriched with Stowaway transposons in potato.

ABSTRACT:

Background

Meiotic recombination is the foundation for genetic variation in natural and artificial populations of eukaryotes. Although genetic maps have been developed for numerous plant species since the late 1980s, few of these maps have provided the necessary resolution needed to investigate the genomic and epigenomic features underlying meiotic crossovers.

Results

Using a whole genome sequencing-based approach, we developed two high-density reference-based haplotype maps using diploid potato clones as parents. The vast majority (81%) of meiotic crossovers were mapped to less than 5 kb. The fine-scale accuracy of crossover detection was validated by Sanger sequencing for a subset of ten crossover events. We demonstrate that crossovers reside in genomic regions of "open ch

SUBMITTER: Marand AP 

PROVIDER: S-EPMC5663088 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature