Sequence analysis of cultivated strawberry (Fragaria × ananassa Duch.) using microdissected single somatic chromosomes.

ABSTRACT:

Background

Cultivated strawberry (Fragaria × ananassa Duch.) has homoeologous chromosomes because of allo-octoploidy. For example, two homoeologous chromosomes that belong to different sub-genome of allopolyploids have similar base sequences. Thus, when conducting de novo assembly of DNA sequences, it is difficult to determine whether these sequences are derived from the same chromosome. To avoid the difficulties associated with homoeologous chromosomes and demonstrate the possibility of sequencing allopolyploids using single chromosomes, we conducted sequence analysis using microdissected single somatic chromosomes of cultivated strawberry.

Results

Three hundred and ten somatic chromosomes of the Japanese octoploid strawberry 'Reiko' were individually selected

SUBMITTER: Yanagi T 

PROVIDER: S-EPMC5663147 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature