Cross platform analysis of transcriptomic data identifies ageing has distinct and opposite effects on tendon in males and females.

ABSTRACT: The development of tendinopathy is influenced by a variety of factors including age, gender, sex hormones and diabetes status. Cross platform comparative analysis of transcriptomic data elucidated the connections between these entities in the context of ageing. Tissue-engineered tendons differentiated from bone marrow derived mesenchymal stem cells from young (20-24 years) and old (54-70 years) donors were assayed using ribonucleic acid sequencing (RNA-seq). Extension of the experiment to microarray and RNA-seq data from tendon identified gender specific gene expression changes highlighting disparity with existing literature and published pathways. Separation of RNA-seq data by sex revealed underlying negative binomial distributions which increased statistical power. Sex specific de novo t

SUBMITTER: Pease LI 

PROVIDER: S-EPMC5663855 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature