The Tiam1 guanine nucleotide exchange factor is auto-inhibited by its pleckstrin homology coiled-coil extension domain.

ABSTRACT: T-cell lymphoma invasion and metastasis 1 (Tiam1) is a Dbl-family guanine nucleotide exchange factor (GEF) that specifically activates the Rho-family GTPase Rac1 in response to upstream signals, thereby regulating cellular processes including cell adhesion and migration. Tiam1 contains multiple domains, including an N-terminal pleckstrin homology coiled-coiled extension (PH_n-CC-Ex) and catalytic Dbl homology and C-terminal pleckstrin homology (DH-PH_c) domain. Previous studies indicate that larger fragments of Tiam1, such as the region encompassing the N-terminal to C-terminal pleckstrin homology domains (PH_n-PH_c), are auto-inhibited. However, the domains in this region responsible for inhibition remain unknown. Here, we show that the PH_n

SUBMITTER: Xu Z 

PROVIDER: S-EPMC5663878 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature