A total transcriptome profiling method for plasma-derived extracellular vesicles: applications for liquid biopsies.

ABSTRACT: Extracellular vesicles (EVs) are key mediators of intercellular communication. Part of their biological effects can be attributed to the transfer of cargos of diverse types of RNAs, which are promising diagnostic and prognostic biomarkers. EVs found in human biofluids are a valuable source for the development of minimally invasive assays. However, the total transcriptional landscape of EVs is still largely unknown. Here we develop a new method for total transcriptome profiling of plasma-derived EVs by next generation sequencing (NGS) from limited quantities of patient-derived clinical samples, which enables the unbiased characterization of the complete RNA cargo, including both small- and long-RNAs, in a single library preparation step. This approach was applied to RNA extracted from EVs i

SUBMITTER: Amorim MG 

PROVIDER: S-EPMC5663969 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature